Gas-liquid Mass Transfer in Elevated Viscosity Medium with Corynebacterium glutamicum DM 1730 Culture Medium

Abstract:
Screening for the best microorganisms for further fermentation in Respiration Activity Monitoring System (RAMOS) is useful and crucial since oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), and respiration quotient (RQ) can be monitored online. Dimensionless number, phase number, describing ‘in phase’ and ‘out-of-phase’ phenomena are used for characterizing fermentation. The gas-liquid mass transfer was investigated using a sodium sulphite system and also a biological system employing Corynebacterium glutamicum DM 1730. All experiments conducted under oxygen limitation conditions described the observed growth rate of a function of mass transfer rate alone. A maximum gas-liquid mass transfer capacity OTRmax was investigated under the biological system with C. glutamicum DM 1730 culture medium prepared at different viscosities ranging from approx. 1 – 63 mPa s using polyvinylpyrrolidone (PVP) and from approx. 1 – 24 mPa s using carboxymethyl cellulose (CMC). In a sulphite system, the volumetric gas-liquid mass transfer coefficient kLa was calculated at different conditions with PVP at viscosities ranging from approx. 1 – 240 mPa s. Both cases, in sulphite system and biological systems indicated an increase of OTRmax with the increase of viscosities. Nevertheless, these were only indicated until certain range of viscosities of 10 – 20 mPa s.

Keywords:
volumetric gas-liquid mass transfer coefficient, PVP, oxygen transfer rate

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