Carsten Bäumchen, Arndt Knoll, Bernward Husemann, Juri Seletzky, Bernd Maier, Carsten Dietrich, Ghassem Amoabediny, Jochen Büchs:
Effect of elevated dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum on D-glucose and L-lactate
Journal of Biotechnology, 2007, 128(4), 868-874
The effect of increased dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum was studied with continuous turbidostatic cultures. The carbon sources were either L-lactate or D-glucose. To increase the dissolved carbon dioxide concentration the carbon dioxide partial pressure of the inlet gas stream p(CO2), (IN) was increased stepwise from 0.0003 bar (air) up to 0.79 bar, while the oxygen partial pressure of the inlet gas stream was kept constant at 0.21 bar. For each resulting carbon dioxide partial pressure p(CO2) the maximum specific growth rate mu(max) was determined from the feed rate resulting from the turbidostatic control. On D-glucose and p(CO2) up to 0.26 bar, mu(max) was mostly constant around 0.58 h(-1). Higher p(CO2) led to a slight decrease of mu(max). On L-lactate mu(max) increased gradually with increasing carbon dioxide partial pressures from 0.37 h(-1) under aeration with air to a maximum value of 0.47 h(-1) at a p(CO2) of 0.26 bar. At very high p(CO2) (0.81 bar) mu(max) decreased down to 0.35 h(-1) independent of the carbon source. (c) 2007 Elsevier B.V. All rights reserved.
corynebacterium glutamicum, dissolved carbon dioxide, carbon dioxide sensitivity, carbon dioxide inhibition, turbidostat culture, pressure fermentation, high cell density cultivation (carboxylases, anaplerotic reactions)