Feasibility of gas/solid carboligation: conversion of benzaldehyde to benzoin using thiamine diphosphate dependent enzymes

Abstract:
A carboligation was investigated for the first time as an enzymatic gas phase reaction, where benzaldehyde was converted to benzoin using thiamine diphosphate (ThDP)-dependent enzymes, namely benzaldehyde lyase (BAL) and benzoylformate decarboxylase (BFD). The biocatalyst was immobilized per deposition on non-porous support. Some limitations of the gas/solid biocatalysis are discussed based on this carboligation and it is also demonstrated that the solid/gas system is an interesting tool for more volatile products.

Keywords:
carboligation, solid/gas bioreactor, enzymatic catalysis, immobilized enzyme, benzoin formation, thiamine diphosphate-dependent enzymes

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