Thomas Drepper, Robert Huber, Achim Heck, Franco Circolone, Ann-Kathrin Hillmer, Jochen Büchs, Karl-Erich Jaeger:
Flavin Mononucleotide-based fluorescent reporter proteins outperform GFP-like proteins as quantitative in vivo real-time reporters
Applied and Environmental Microbiology, 2010, 76(17), 5990-5994
Fluorescent proteins of the green fluorescent protein (GFP) family are commonly used as reporter proteins for quantitative analysis of complex biological processes in living microorganisms. Here we demonstrate that the fluorescence signal intensity of GFP-like proteins is affected under oxygen limitation and therefore does not reflect the amount of reporter protein in Escherichia coli batch cultures. Instead, flavin mononucleotide (FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under these conditions.