BioVT-2010-20 [BibTeX]
Ghassem Amoabediny, Jochen Büchs:
Determination of CO2 sensitivity of micro-organisms in shaken bioreactors. I. Novel method based on the resistance of sterile closure
Biotechnology and Applied Biochemistry, 2010, 57(4), 157-166
Abstract:
Influence of carbon dioxide on growth and product kinetics
of industrially important micro-organisms is essential
for the interpretation of a bioprocess. In this research,
the CO2 effects on productivity and growth rate
of micro-organisms have been studied by using a variety
of kplug. The applied method is based on a different concentration
of CO2 in the headspace of ventilation flasks.
The presented method is simple, inexpensive and
shows similar results compared to large-scale fermentation
regarding the evolution of CO2 in a batch system.
For the investigation of the proposed method, experiments
employing Arxula adeninivorans LS3, Corynebacterium
glutamicum (DM1730 and ATCCWT13032) and
Hansenula polymorpha DSM70277 as model organisms
in the ventilation flasks are performed. The fermentations
in the RAMOS (respiratory activity monitoring
system) device were carried out with a normal aeration
rate (1 vvm) and under the same operating conditions
as the ventilation flask f1. The modified unsteady-state
model was used to predict the operation conditions of a
biological system in the ventilation flasks. In the present
study, a novel and easy method for the quantification
of CO2 sensitivity of micro-organisms in shaken
bioreactors (called ventilation flask) was achieved.
Keywords:
biomass, bioreactor, carbon dioxide, fermentation, unsteady model, ventilation flask