BioVT-2010-17 BibTeX
@ARTICLE{BioVT-2010-17,
AUTHOR = {Thomas Drepper and Robert Huber and Achim Heck and Franco Circolone and Ann-Kathrin Hillmer and J. B\"{u}chs and Karl-Erich Jaeger},
TITLE = {{Flavin Mononucleotide-based fluorescent reporter proteins outperform GFP-like proteins as quantitative in vivo real-time reporters}},
JOURNAL = {Applied and Environmental Microbiology},
YEAR = {2010},
volume = {76},
number = {17},
pages = {5990-5994},
month = {},
note = {},
abstract = {Fluorescent proteins of the green fluorescent protein (GFP) family are commonly used as reporter proteins
for quantitative analysis of complex biological processes in living microorganisms. Here we demonstrate that
the fluorescence signal intensity of GFP-like proteins is affected under oxygen limitation and therefore does not
reflect the amount of reporter protein in Escherichia coli batch cultures. Instead, flavin mononucleotide
(FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under these
conditions.},
keywords = {},
}
Thomas Drepper, Robert Huber, Achim Heck, Franco Circolone, Ann-Kathrin Hillmer, Jochen Büchs, Karl-Erich Jaeger:
Flavin Mononucleotide-based fluorescent reporter proteins outperform GFP-like proteins as quantitative in vivo real-time reporters
Applied and Environmental Microbiology, 2010, 76(17), 5990-5994
Abstract:
Fluorescent proteins of the green fluorescent protein (GFP) family are commonly used as reporter proteins
for quantitative analysis of complex biological processes in living microorganisms. Here we demonstrate that
the fluorescence signal intensity of GFP-like proteins is affected under oxygen limitation and therefore does not
reflect the amount of reporter protein in Escherichia coli batch cultures. Instead, flavin mononucleotide
(FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under these
conditions.
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