BioVT-2011-09 BibTeX
@ARTICLE{BioVT-2011-09,
AUTHOR = {N. Mehmood and E. Olmos and J.-L. Goergen and F. Blanchard and D. Ullisch and W. Kl\"{o}ckner and J. B\"{u}chs and S. Delaunay},
TITLE = {{Oxygen supply controls the onset of pristinamycins production by Streptomyces pristinaespiralis in shaking flasks}},
JOURNAL = {Biotechnology and Bioengineering},
YEAR = {2011},
volume = {108},
number = {9},
pages = {2151-2161},
month = {},
note = {},
abstract = {Antibiotics are secondary metabolites, generally
produced during stationary phase of growth under
different nutritional and hydrodynamic stresses. However,
the exact mechanisms of the induction of antibiotics production
are still not clearly established. In a previous study,
the induction of pristinamycins production by Streptomyces
pristinaespiralis as well as product concentrations were
correlated with power dissipation per unit of volume (P/
V) in shaking flasks. In this study, detailed kinetics of
growth, substrate consumption, oxygen transfer rate and
pristinamycins production under varying P/V conditions
have been obtained and analyzed. Our results showed that
higher P/V resulted in a higher concentration of biomass and
promoted an earlier nutrient limitation and ultimately
an earlier induction of pristinamycins production. The
maximal specific growth rate, specific oxygen consumption
rate and specific consumption rate of glutamate increased
with P/V while influence was less marked with specific
consumption rate of glucose, arginine, ammonium ions
and phosphate. When oxygen uptake rate (OUR) was
limited by free-surface oxygen transfer, pristinamycins production
was not detected despite the occurrence of nitrogen
and/or phosphate sources limitation. The threshold value
for OUR observed was around 25 mmol L1 h1. This suggested
that a limitation in nitrogen and/or phosphate alone
was not sufficient to induce pristinamycins production by
S. pristinaespiralis pr11. To induce this production, the
oxygen transfer had to be non-limiting.},
keywords = {Streptomyces, power dissipation, nutrient limitation, pristinamycins production, shaking flask},
}
N. Mehmood, E. Olmos, J.-L. Goergen, F. Blanchard, David Ullisch, Wolf Klöckner, Jochen Büchs, S. Delaunay:
Oxygen supply controls the onset of pristinamycins production by Streptomyces pristinaespiralis in shaking flasks
Biotechnology and Bioengineering, 2011, 108(9), 2151-2161
Abstract:
Antibiotics are secondary metabolites, generally
produced during stationary phase of growth under
different nutritional and hydrodynamic stresses. However,
the exact mechanisms of the induction of antibiotics production
are still not clearly established. In a previous study,
the induction of pristinamycins production by Streptomyces
pristinaespiralis as well as product concentrations were
correlated with power dissipation per unit of volume (P/
V) in shaking flasks. In this study, detailed kinetics of
growth, substrate consumption, oxygen transfer rate and
pristinamycins production under varying P/V conditions
have been obtained and analyzed. Our results showed that
higher P/V resulted in a higher concentration of biomass and
promoted an earlier nutrient limitation and ultimately
an earlier induction of pristinamycins production. The
maximal specific growth rate, specific oxygen consumption
rate and specific consumption rate of glutamate increased
with P/V while influence was less marked with specific
consumption rate of glucose, arginine, ammonium ions
and phosphate. When oxygen uptake rate (OUR) was
limited by free-surface oxygen transfer, pristinamycins production
was not detected despite the occurrence of nitrogen
and/or phosphate sources limitation. The threshold value
for OUR observed was around 25 mmol L1 h1. This suggested
that a limitation in nitrogen and/or phosphate alone
was not sufficient to induce pristinamycins production by
S. pristinaespiralis pr11. To induce this production, the
oxygen transfer had to be non-limiting.
Keywords:
Streptomyces, power dissipation, nutrient limitation, pristinamycins production, shaking flask