Characterization of ionic liquid assisted enzymatic cellulose hydrolysis
|The efficient hydrolysis of cellulose is a key process step for the economical use of biomass as a raw material. In this work ionic liquids are used for effective cellulose pretreatment and the cellulose is hydrolyzed enzymatically.|
This project is concerned with the mechanistically motivated characterization of enzymatic cellulose depolymerization. Ionic liquids are used as a new generation of solvents for dissolving cellulose and hemicellulose. Online measurement techniques, such as light scattering and fluorescence, allow the use of rapid screening procedures to analyze dissolution characteristics and enzyme activities in µL scale.
A number of different enzyme activities are working together simultaneously during the hydrolysis of cellulose. The aim of this project is to understand individual enzyme mechanisms and kinetics in order to optimize the reaction system and its synergistic effects. Innovative analytical procedures, such as gel permeation chromatography in combination with multi angle light scattering are used to measure absolute molecular weight distribution of the cellulose chains. Changes in the chain length distribution during the enzymatic hydrolysis are used in mechanistic kinetic models of the enzyme reaction using population balances.
|Funded by:||Exzellenz-Cluster „ Tailor-Made Fuels from Biomass“ / DFG|
|Projectmanager:||Lehrstuhl für Bioverfahrenstechnik|
|Contact:||Philip Engel, Helene Wulfhorst, Antje Spieß, Benjamin Bonhage|