Development of a continuous parallel shaken bioreactor system in small scale

  • Entwicklung eines kontinuierlichen, parallel geschüttelten Bioreaktorsystems in kleinem Maßstab

Akgün, Ali; Büchs, Jochen (Thesis advisor)

Aachen : Publikationsserver der RWTH Aachen University (2008)
Dissertation / PhD Thesis

Aachen, Techn. Hochsch., Diss., 2007


Microbiological research is mostly based on cultivation of microorganisms. Continuous-flow culture systems compared to batch operation offers the advantages of constant environmental conditions for biological reactions which can be highly sensitive to process variations (Calcott 1981). Another significant advantage of continuous culture is that the rate of dilution controls the rate of microbial growth via the concentration of the growth-limiting nutrient in the medium. Despite a large number of advantages and many application possibilities, the conventional continuous-flow culture systems are due to their complex set up and large working volume inappropriate for a wide application in the academic disciplines. Therefore, limited knowledge and experience about the continuous-flow cultivation of biological systems set also a significant barrier to use of this technique in industrial applications. Focusing on the operational attractiveness and widespread usage of shaking technology in a number of academic and industrial disciplines, a novel continuous-flow parallel shaken bioreactor system based on shaking technology, exploiting the advantages of shake flasks of easy handling, a parallel applicability, and reduction of overall expenses was investigated. This dissertation describes the principle and the mechanical design of the continuous-flow parallel shaken bioreactor system, as well as the experiments conducted for the technical characterization and biological validation of the novel system.