High-throughput experimentation for microscale cultivations and recombinant protein expression

  • Hochdurchsatz-Versuchsdurchführung für Mikromaßstab-Kulturen und rekombinante Proteinexpression

Huber, Robert; Büchs, Jochen (Thesis advisor)

Aachen : Publikationsserver der RWTH Aachen University (2010)
Dissertation / PhD Thesis

Aachen, Techn. Hochsch., Diss., 2010


In this work, common high-throughput cultivation processes in microtiter plates are investigated with the BioLector technology. It is demonstrated that extensive monitoring of key parameters (i.e. biomass and product formation) in microscale cultivations enables a profound understanding and hence optimization of recombinant expression systems and high-throughput cultivation processes. New concepts to deal with issues of different growth kinetics of cultures in microtiter plates are elaborated and tested. Furthermore, a novel high-throughput experimentation platform for microscale cultivations is established by combining a pipetting robot with the BioLector, resulting in the Robo-Lector platform. Ultimately, the Robo-Lector can contribute to the envisioned paradigm shift in bioprocess development. This entails switching from rather empirical process development (low-throughput experimentation with no or unsophisticated monitoring) to high-throughput experimentation with very sophisticated yet simple monitoring to gain deeper knowledge of biological production systems.