InterTwined – Process development studies towards fungal-bacterial co-cultivations
- Biochemical Engineering
- Masterthesis / Bachelorthesis / Forschungspraktikum for Biotechnologen
- Focus/Key Topic:
Defined fermentation conditions possess many advantages considering process control and the subsequent downstream. Most often, these conditions are incomparable with natural habitats. For example, pure cultures solely exist within the laboratory. Missing interactions lead to a stunted metabolic profile and silent gene-clusters. Moreover, mixed cultures are beneficial concerning substrate availability and are needed for further process integration.
Therefore, this project takes a step towards uncovering these advantages. The aim is a defined co-cultivation of the filamentous fungus Trichoderma reesei and a filamentous bacteria e.g Streptomyces coelicolor. Insoluble cellulose serves as substrate, which is hydrolyzed by secreted cellulases of T. reesei. Hence, glucose is made available to ensure growth and secondary metabolite production. The antibiotics actinorhodin and undecylprodigiosin produced by S. coelicolor are of particular interest. By means of co-cultivation an enhanced production and the search for further natural products is targeted.
The complexity of this project enables us to delve into different topics based on your interest. With our state of the art technology, you could conduct studies regarding growth characterization, limitation induced product formation, morphology dependent viscosity, pellet formation or modelling of different phenomena.
This work offers you …
- … a broad understanding of biotechnological processes
- … an introduction into a seminal topic with the flexibility to introduce your own ideas
- … a dedicated supervision
This work requires …
- … motivation and perseverance to dig deep into challenging topics
- … enthusiasm and accuracy especially for laboratory work
- … capabilities to think outside the box
If you are interested, do not hesitate to contact me. Whether in English or German, I would gladly give you more details in a personal talk.